Assay Name
dPCR Mutation Assay WT1 27370 Human
GeneGlobe Cat No (Assay ID)
DMH0000048
Species
Human (Homo sapiens)
Gene Symbol
WT1
Gene aliases
WAGR;WIT-2;AWT1;NPHS4;WT-1;GUD
Ensembl Gene ID
ENSG00000184937
Entrez Gene ID
7490
Genomic Mutation ID (COSV by COSMIC)
COSV60066459
Legacy Mutation ID (COSM by COSMIC)
COSM27370
Amino Acid Change
p.D464N
Nucleotide Change
c.1390G>A
Wildtype Allele
C
Mutant Allele
T
Mutation Strand
-
Mutant description
Substitution - Missense
Positive Control Sequence
GCACTTGTTTTACCTGTATGAGTCCTGGTGTGGGTCTTCAGGTGGTCGGACCGGGAGAACTTTCGCTGACAAGTTTTACACTGGAATGGTTTCACA
Amplicon length
76
Recommended Restriction Enzyme
CviQI,AluI,HaeIII,EcoRI,XbaI,PvuII
Wet-lab validated
dPCR wet-lab validated
Probe Fluorophore
FAM/HEX or ATTO550/ROX
Quencher
Iowa Black
Primer Purification
Desalted
Probe Purification
HPLC