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Products | QIAseq Targeted DNA Pro Custom PanelFor unprecedented ease-of-use and biological insights using targeted DNA sequencing | QIAseq Targeted cfDNA Ultra Custom PanelFor ultrasensitive targeted next-generation sequencing of cfDNA on Illumina platforms | QIAseq Targeted DNA Custom PanelsDigital DNA sequencing to confidently detect low-frequency variants | QIAseq xHYB Custom Human PanelA flexible customization, hybrid-capture-based solution to enable sample-to-insight, targeted sequencing of DNA by next-generation sequencing | QIAseq Targeted Methyl Custom PanelsA liquid biopsy-compatible solution for targeting methylation status of DNA, from as low as 1 ng input DNA from cells and tissues, or 10 ng from FFPE samples and liquid biopsies |
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Application | DNA variant detection | cfDNA variant detection | DNA variant detection | DNA variant detection | Methylation status analysis |
Analyte | DNA (including cfDNA, ctDNA) | cfDNA, ctDNA only | DNA (including cfDNA, ctDNA) | DNA (including cfDNA, ctDNA) | DNA (including cfDNA, ctDNA) |
Sample Input | 10–200 ng | 5–80 ng | 10–200 ng | 20–1000 ng | 1–100 ng fresh DNA, 10–200 ng FFPE DNA, or 10–200 ng ccfDNA |
Supported Species | Human | Human | Human | Human | Human |
Reference Genome | Human GRCh38 | Human GRCh38 | Human GRCh37/hg19 | Human GRCh38 | Human GRCh38/hg38 and GRCh37/hg19 |
Supported Platforms | Platform-agnostic solution | Illumina | Platform-agnostic solution | Platform-agnostic solution | Illumina |
Number of Samples | 96 | 96 | 96 | 96 | 96, 384 |
Design Type | New design (own data), or boost a QIAGEN or your own predesigned panel | New design (own data), or boost a QIAGEN or your own predesigned panel | New design (own data), or boost a QIAGEN or your own predesigned panel | New design (own data), or boost a QIAGEN or your own predesigned panel | New design (own data), or boost a QIAGEN or your own predesigned panel |
Other Features | UMI, UDI | UMI, UDI | UMI, UDI | dsDNA probes | UMI |
Data Analysis Solution | Variant calling using GeneGlobe Data Analysis Portal; variant calling using CLC Genomics Workbench | CLC Genomics Workbench | GeneGlobe Data Analysis Portal, CLC Genomics Workbench | CLC Genomics Workbench | GeneGlobe Data Analysis Portal, CLC Genomics Workbench |
Technology | QIAseq enrichment technology (one target-specific primer and one universal primer) | QIAseq enrichment technology (one target-specific primer and one universal primer) | QIAseq enrichment technology (one target-specific primer and one universal primer) | Hybrid capture | QIAseq enrichment technology (one target-specific primer and one universal primer) |
Products | QIAseq Targeted RNA Custom PanelsFor highly accurate gene expression quantification using UMIs | QIAseq UPX 3' Targeted RNA PanelsFor ultraplex (UPX) gene expression using targeted 3' RNA-seq from cell lysates and up to 10ng of RNA | QIAseq FastSelect Custom RNA Removal KitFor rapid removal of unwanted RNAs during RNA-seq library preparation | QIAseq Fusion XP Custom PanelsComprehensive oncology profiling combining gene fusion detection, gene expression analysis and single nucleotide variant (SNV) calling in just one assay |
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Application | Quantitative gene expression profiling | 3' RNAseq from low-input samples | Removal of unwanted RNA from RNA-seq libraries | Gene fusion, gene expression, exon skipping and RNA SNV/InDel analysis |
Analyte | RNA | RNA | RNA | RNA |
Sample Input | 25 ng to 5 μg; Less than 25 ng with low-input protocol | Cell lysate and isolated RNA from 10 pg to 10 ng per sample | Flexibility to use RNA from FFPE samples or degraded RNA samples, or high-quality RNA | Difficult sample types such as FFPE, isolated total RNA (10 ng or even less) |
Supported Species | Human, mouse, rat | Human, mouse | All | Human |
Reference Genome | Human GRCh38, Mouse GRCm38, Rat RGCS6.0/rn6 | Ensemble V87 human: GRCh38.p2; mouse: GRCm38.p5 | N/A | Human GRCh38/hg38 |
Supported Platforms | Illumina and Thermo-Fisher NGS instruments | Illumina NGS instruments | QIAGEN, Illumina, NEB, KAPA and other stranded RNA-seq library kits | Illumina and Thermo-Fisher NGS instruments |
Number of Samples | 12, 96, 384 | 96, 4x96, 384 | 1536 | 96 |
Design Type | New design or extend a predesigned panel | Custom panel of 12 to 1000 genes | Specify up to 20,000 bases of RNAs for removal | New design (own data), Boost own or QIAGEN product |
Other Features | UMI | UMI, UPX | UMI, UDI | |
Data Analysis Solution | Access to GeneGlobe Data Analysis Portal (including read alignment and data normalization, differential gene expression) at no additional costs | Includes cloud-based read alignment | Analyze RNA-seq data with ease using the GeneGlobe-integrated RNA-seq Analysis Portal – an intuitive, web-based data analysis solution created for biologists and included with this QIAseq Kit | GeneGlobe Data Analysis Portal, CLC Genomics Workbench |
Technology | 2-primer amplicon-based (single amplicon per gene) | Pooled cDNA uses 2 primer amplicon consisting of a universal primer and transcript specific primer | QIAseq enrichment technology (one target-specific primer and one universal primer) |
Products | QIAseq Targeted RNA Custom PanelsFor highly accurate gene expression quantification using UMIs | QIAseq UPX 3' Targeted RNA PanelsFor ultraplex (UPX) gene expression using targeted 3' RNA-seq from cell lysates and up to 10ng of RNA | QIAseq FastSelect Custom RNA Removal KitFor rapid removal of unwanted RNAs during RNA-seq library preparation |
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Application | Quantitative gene expression profiling | 3' RNAseq from low-input samples | Removal of unwanted RNA from RNA-seq libraries |
Analyte | RNA | RNA | RNA |
Sample Input | 25 ng to 5 μg; Less than 25 ng with low-input protocol | Cell lysate and isolated RNA from 10 pg to 10 ng per sample | Flexibility to use RNA from FFPE samples or degraded RNA samples, or high-quality RNA |
Supported Species | Human, mouse, rat | Human, mouse | All |
Reference Genome | Human GRCh38, Mouse GRCm38, Rat RGCS6.0/rn6 | Ensemble V87 human: GRCh38.p2; mouse: GRCm38.p5 | N/A |
Supported Platforms | Illumina and Thermo-Fisher NGS instruments | Illumina NGS instruments | QIAGEN, Illumina, NEB, KAPA and other stranded RNA-seq library kits |
Number of Samples | 12, 96, 384 | 96, 4x96, 384 | 1536 |
Design Type | New design or extend a predesigned panel | Custom panel of 12 to 1000 genes | Specify up to 20,000 bases of RNAs for removal |
Other Features | UMI | UMI, UPX | |
Data Analysis Solution | Access to GeneGlobe Data Analysis Portal (including read alignment and data normalization, differential gene expression) at no additional costs | Includes cloud-based read alignment | Analyze RNA-seq data with ease using the GeneGlobe-integrated RNA-seq Analysis Portal – an intuitive, web-based data analysis solution created for biologists and included with this QIAseq Kit |
Technology | 2-primer amplicon-based (single amplicon per gene) | Pooled cDNA uses 2 primer amplicon consisting of a universal primer and transcript specific primer |
Products?????? | QIAseq Targeted RNA Custom PanelsFor highly accurate gene expression quantification using UMIs | QIAseq FastSelect Custom RNA Removal KitFor rapid removal of unwanted RNAs during RNA-seq library preparation |
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Application | Quantitative gene expression profiling | Removal of unwanted RNA from RNA-seq libraries |
Analyte | RNA | RNA |
Sample Input | 25 ng to 5 μg; Less than 25 ng with low-input protocol | Flexibility to use RNA from FFPE samples or degraded RNA samples, or high-quality RNA |
Supported Species | Human, mouse, rat | All |
Reference Genome | Human GRCh38, Mouse GRCm38, Rat RGCS6.0/rn6 | N/A |
Supported Platforms | Illumina and Thermo-Fisher NGS instruments | QIAGEN, Illumina, NEB, KAPA and other stranded RNA-seq library kits |
Number of Samples | 12, 96, 384 | 1536 |
Design Type | New design or extend a predesigned panel | Specify up to 20,000 bases of RNAs for removal |
Other Features | UMI | |
Data Analysis Solution | Access to GeneGlobe Data Analysis Portal (including read alignment and data normalization, differential gene expression) at no additional costs | Analyze RNA-seq data with ease using the GeneGlobe-integrated RNA-seq Analysis Portal – an intuitive, web-based data analysis solution created for biologists and included with this QIAseq Kit |
Technology | 2-primer amplicon-based (single amplicon per gene) |
Products | QIAseq FastSelect Custom RNA Removal KitFor rapid removal of unwanted RNAs during RNA-seq library preparation |
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Application | Removal of unwanted RNA from RNA-seq libraries |
Analyte | RNA |
Sample Input | Flexibility to use RNA from FFPE samples or degraded RNA samples, or high-quality RNA |
Supported Species | All |
Reference Genome | N/A |
Supported Platforms | QIAGEN, Illumina, NEB, KAPA and other stranded RNA-seq library kits |
Number of Samples | 1536 |
Design Type | Specify up to 20,000 bases of RNAs for removal |
Other Features | |
Data Analysis Solution | Analyze RNA-seq data with ease using the GeneGlobe-integrated RNA-seq Analysis Portal – an intuitive, web-based data analysis solution created for biologists and included with this QIAseq Kit |
Technology |
Products | QIAseq FastSelect Custom RNA Removal KitFor rapid removal of unwanted RNAs during RNA-seq library preparation |
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Application | Removal of unwanted RNA from RNA-seq libraries |
Analyte | RNA |
Sample Input | Flexibility to use RNA from FFPE samples or degraded RNA samples, or high-quality RNA |
Supported Species | All |
Reference Genome | N/A |
Supported Platforms | QIAGEN, Illumina, NEB, KAPA and other stranded RNA-seq library kits |
Number of Samples | 1536 |
Design Type | Specify up to 20,000 bases of RNAs for removal |
Other Features | |
Data Analysis Solution | Analyze RNA-seq data with ease using the GeneGlobe-integrated RNA-seq Analysis Portal – an intuitive, web-based data analysis solution created for biologists and included with this QIAseq Kit |
Technology |
Products | QIAseq Multimodal HT Custom PanelsA single day sample to sequencing workflow for simultaneous enrichment and profiling of DNA variants, RNA fusions and gene expression levels from one sample input, as low as 10 ng |
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Application | DNA: SNVs, InDels (also available: TMB/MSI); RNA: gene fusion, exon skipping, gene expression, SNVs, InDels |
Analyte | DNA, RNA, DNA & RNA |
Sample Input | DNA: 10–40 ng for fresh samples or 40–250 ng for FFPE; RNA: 10–250 ng for fresh samples or 100–250 ng for FFPE |
Supported Species | Human |
Reference Genome | DNA module: GRCh37/hg19; RNA module: GRCh38 |
Supported Platforms | Illumina, compatible with other platforms |
Number of Samples | 96 |
Design Type | New design (own data), or boost a QIAGEN or your own predesigned panel |
Other Features | UMI, UDI |
Data Analysis Solution | GeneGlobe Data Analysis Portal, CLC Genomics Workbench |
Technology | QIAseq enrichment technology (one target-specific primer and one universal primer) |
Want to take the stress out of data analysis and fast-track your path to gene expression insights? With our new RNA-seq Analysis Portal, go from FASTQ files to pathway analysis insights in hours instead of days, integrating your data with qPCR and dPCR verification.
Easy. Fast. Online. Our portal supports the analyses for 18 different species using proven published algorithms. The RNA-seq Analysis Portal is compatible with QIAseq and other major RNA library kits. Perform your analyses anytime, anywhere.