Assay Name
dPCR Mutation Assay DNMT3A 52944 Human
GeneGlobe Cat No (Assay ID)
DMH0000489
Species
Human (Homo sapiens)
Gene Symbol
DNMT3A
Ensembl Gene ID
ENSG00000119772
Entrez Gene ID
1788
Genomic Mutation ID (COSV by COSMIC)
COSV53036153
Legacy Mutation ID (COSM by COSMIC)
COSM52944
Amino Acid Change
p.R882H
Nucleotide Change
c.2645G>A
Wildtype Allele
C
Mutant Allele
T
Mutation Strand
-
Mutant description
Substitution - Missense
Positive Control Sequence
AAGAGGTGGCGGATGACTGGCACGCTCCATGACCGGCCCAGCAGTCTCTGCCTCGCCAAGCGGCTCATGTTGGAGACGTCAGTATAGTGGACTGGGAAACCAAATACCCTGGGGGA
Amplicon length
96
Recommended Restriction Enzyme
CviQI,AluI,EcoRI,XbaI,PvuII
Wet-lab validated
dPCR wet-lab validated
Probe Fluorophore
FAM/HEX or ATTO550/ROX
Quencher
Iowa Black
Primer Purification
Desalted
Probe Purification
HPLC