Assay Name
dPCR Mutation Assay FLT3 116249 Human
GeneGlobe Cat No (Assay ID)
DMH0000009
Species
Human (Homo sapiens)
Gene Symbol
FLT3
Gene aliases
STK1;FLK2;CD135
Ensembl Gene ID
ENSG00000122025
Entrez Gene ID
2322
Genomic Mutation ID (COSV by COSMIC)
COSV54042664
Legacy Mutation ID (COSM by COSMIC)
COSM116249
Amino Acid Change
p.V491L
Nucleotide Change
c.1471G>C
Wildtype Allele
C
Mutant Allele
G
Mutation Strand
-
Mutant description
Substitution - Missense
Positive Control Sequence
CACAGCACTTGACCAGGAACCCTTTTATGGCTTCACTCATGTTTAGAGTACTGCTCGACACCCACTGTCCAAACACTTTTCTGTTAGCCTTTCTATTCCAGACTCCTTCTGTGA
Amplicon length
94
Recommended Restriction Enzyme
AluI,HaeIII,EcoRI,XbaI,PvuII
Wet-lab validated
dPCR wet-lab validated
Probe Fluorophore
FAM/HEX or ATTO550/ROX
Quencher
Iowa Black
Primer Purification
Desalted
Probe Purification
HPLC