Assay Name
dPCR Mutation Assay IDH1 28750 Human
GeneGlobe Cat No (Assay ID)
DMH0000015
Species
Human (Homo sapiens)
Gene Symbol
IDH1
Ensembl Gene ID
ENSG00000138413
Entrez Gene ID
3417
Genomic Mutation ID (COSV by COSMIC)
COSV61615420
Legacy Mutation ID (COSM by COSMIC)
COSM28750
Amino Acid Change
p.R132L
Nucleotide Change
c.395G>T
Wildtype Allele
C
Mutant Allele
A
Mutation Strand
-
Mutant description
Substitution - Missense
Positive Control Sequence
TCACATTATTGCCAACATGACTTACTTGATCCCCATAAGCATGACGACCTATGATGATAGGTTTTACCCATCCACTCACAAGCCGG
Amplicon length
66
Recommended Restriction Enzyme
CviQI,AluI,HaeIII,EcoRI,XbaI,PvuII
Wet-lab validated
dPCR wet-lab validated
Probe Fluorophore
FAM/HEX or ATTO550/ROX
Quencher
Iowa Black
Primer Purification
Desalted
Probe Purification
HPLC