Assay Name
dPCR Mutation Assay WT1 27307 Human
GeneGlobe Cat No (Assay ID)
DMH0000060
Species
Human (Homo sapiens)
Gene Symbol
WT1
Gene aliases
WAGR;WIT-2;AWT1;NPHS4;WT-1;GUD
Ensembl Gene ID
ENSG00000184937
Entrez Gene ID
7490
Genomic Mutation ID (COSV by COSMIC)
COSV60065297
Legacy Mutation ID (COSM by COSMIC)
COSM27307
Amino Acid Change
p.S381*
Nucleotide Change
c.1142C>A
Wildtype Allele
G
Mutant Allele
T
Mutation Strand
-
Mutant description
Substitution - Missense
Positive Control Sequence
CTTATTGCAGCCTGGGTAAGCACACATGAAGGGGCGTTTCTCACTGGTCTCAGATGCCGACCGTACAAGAGTCGGGGCTACTCCAGGCACACGTCGCACATCCTGCAGGCAG
Amplicon length
92
Recommended Restriction Enzyme
AluI,HaeIII,EcoRI,XbaI,PvuII
Wet-lab validated
dPCR wet-lab validated
Probe Fluorophore
FAM/HEX or ATTO550/ROX
Quencher
Iowa Black
Primer Purification
Desalted
Probe Purification
HPLC