Assay Name
dPCR Mutation Assay EGFR 12419 Human
GeneGlobe Cat No (Assay ID)
DMH0000075
Species
Human (Homo sapiens)
Gene Symbol
EGFR
Gene aliases
ERBB1;ERRP;ERBB
Ensembl Gene ID
ENSG00000146648
Entrez Gene ID
1956
Genomic Mutation ID (COSV by COSMIC)
COSV51863059
Legacy Mutation ID (COSM by COSMIC)
COSM12419
Amino Acid Change
p.L747_T751delinsQ
Nucleotide Change
c.2238_2252delinsGCA
Wildtype Allele
AATTAAGAGAAGCAAC
Mutant Allele
AGCA
Mutation Strand
+
Mutant description
Substitution - Missense
Positive Control Sequence
TAGGGACTCTGGATCCCAGAAGGTGAGAAAGTTAAAATTCCCGTCGCTATCAAGGAATTAAGAGAAGCAACATCTCCGAAAGCCAACAAGGAAATCCTCGATGTGAGTTTCTGCTTTGCTGTGTGGGGG
Amplicon length
109
Recommended Restriction Enzyme
CviQI,AluI,HaeIII,EcoRI,XbaI,PvuII
Wet-lab validated
dPCR wet-lab validated
Probe Fluorophore
FAM/HEX or ATTO550/ROX
Quencher
Iowa Black
Primer Purification
Desalted
Probe Purification
HPLC