Assay Name
dPCR Mutation Assay MET 735 Human
GeneGlobe Cat No (Assay ID)
DMH0000256
Species
Human (Homo sapiens)
Gene Symbol
MET
Gene aliases
HGFR;RCCP2;DFNB97
Ensembl Gene ID
ENSG00000105976
Entrez Gene ID
4233
Genomic Mutation ID (COSV by COSMIC)
COSV59264428
Legacy Mutation ID (COSM by COSMIC)
COSM735
Amino Acid Change
p.?
Nucleotide Change
c.3083-52_3083-51insCT
Wildtype Allele
C
Mutant Allele
CCT
Mutation Strand
+
Mutant description
Substitution - Missense
Positive Control Sequence
TTTTTATCAAACTAATTCCATTATAAAAGCTCTTCCTGTTTCAGTCCCCATTAAATGAGGTTTTACTGTTGTTCTTTAATAATTTTCCTTCATCTTACAGATCAGTTTCCTAATTCATCTCAGAACGGTTCATGCCGACAAGTGCAG
Amplicon length
127
Recommended Restriction Enzyme
CviQI,HaeIII,EcoRI,XbaI,PvuII
Wet-lab validated
dPCR wet-lab validated
Probe Fluorophore
FAM/HEX or ATTO550/ROX
Quencher
Iowa Black
Primer Purification
Desalted
Probe Purification
HPLC