Assay Name
dPCR Mutation Assay ABL1 12576 Human
GeneGlobe Cat No (Assay ID)
DMH0000571
Species
Human (Homo sapiens)
Gene Symbol
ABL1
Gene aliases
JTK7;c-ABL;p150;ABL
Ensembl Gene ID
ENSG00000097007
Entrez Gene ID
25
Genomic Mutation ID (COSV by COSMIC)
COSV59325175
Legacy Mutation ID (COSM by COSMIC)
COSM12576
Amino Acid Change
p.Y272H
Nucleotide Change
c.814T>C
Wildtype Allele
T
Mutant Allele
C
Mutation Strand
+
Mutant description
Substitution - Missense
Positive Control Sequence
TGGAACGCACGGACATCACCATGAAGCACAAGCTGGGCGGGGGCCAGTACGGGGAGGTGTACGAGGGCGTGTGGAAGAAATACAGCCTGACGGTGGCCGT
Amplicon length
80
Recommended Restriction Enzyme
EcoRI,XbaI,PvuII
Wet-lab validated
dPCR wet-lab validated
Probe Fluorophore
FAM/HEX or ATTO550/ROX
Quencher
Iowa Black
Primer Purification
Desalted
Probe Purification
HPLC