Assay Name
dPCR Mutation Assay NRAS 586 Human
GeneGlobe Cat No (Assay ID)
DMH0000180
Species
Human (Homo sapiens)
Gene Symbol
NRAS
Gene aliases
N-ras
Ensembl Gene ID
ENSG00000213281
Entrez Gene ID
4893
Genomic Mutation ID (COSV by COSMIC)
COSV54736320
Legacy Mutation ID (COSM by COSMIC)
COSM586
Amino Acid Change
p.Q61H
Nucleotide Change
c.183A>C
Wildtype Allele
T
Mutant Allele
G
Mutation Strand
-
Mutant description
Substitution - Missense
Positive Control Sequence
AGAGGAAGCCTTCGCCTGTCCTCATGTATTGGTCTCTCATGGCACTGTACTCTTCTTGTCCAGCTGTATCCAGTATGTCCAACAAACAGGTTTCACCATCTATAACCAC
Amplicon length
89
Recommended Restriction Enzyme
HaeIII,EcoRI,XbaI
Wet-lab validated
dPCR wet-lab validated
Probe Fluorophore
FAM/HEX or ATTO550/ROX
Quencher
Iowa Black
Primer Purification
Desalted
Probe Purification
HPLC