Assay Name
dPCR Mutation Assay KRAS 550 Human
GeneGlobe Cat No (Assay ID)
DMH0000044
Species
Human (Homo sapiens)
Gene Symbol
KRAS
Gene aliases
KRAS1;K-Ras4B;KRAS2
Ensembl Gene ID
ENSG00000133703
Entrez Gene ID
3845
Genomic Mutation ID (COSV by COSMIC)
COSV55502677
Legacy Mutation ID (COSM by COSMIC)
COSM550
Amino Acid Change
p.Q61E
Nucleotide Change
c.181C>G
Wildtype Allele
G
Mutant Allele
C
Mutation Strand
-
Mutant description
Substitution - Missense
Positive Control Sequence
CCCTCCCCAGTCCTCATGTACTGGTCCCTCATTGCACTGTACTCCTCTTGACCTGCTGTGTCGAGAATATCCAAGAGACAGGTTTCTCCATCAATTACTA
Amplicon length
80
Recommended Restriction Enzyme
AluI,HaeIII,EcoRI,XbaI,PvuII
Wet-lab validated
dPCR wet-lab validated
Probe Fluorophore
FAM/HEX or ATTO550/ROX
Quencher
Iowa Black
Primer Purification
Desalted
Probe Purification
HPLC