GeneGlobe ID: DMH0000519 | Cat. No.: | dPCR LNA Mutation Assays

dPCR Mutation Assay KRAS 517 Human

AA Mutation: p.G12S
Nucleotide Mutation: c.34G>A
dPCR wet-lab validated

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Product Specification

Assay Name​
dPCR Mutation Assay KRAS 517 Human
GeneGlobe Cat No (Assay ID)​
DMH0000519
Species​
Human (Homo sapiens)
Gene Symbol​
KRAS
Gene aliases​
KRAS1;K-Ras4B;KRAS2
Ensembl Gene ID​
ENSG00000133703
Entrez Gene ID​
3845
Genomic Mutation ID (COSV by COSMIC)​
COSV55497461
Legacy Mutation ID (COSM by COSMIC)​
COSM517
Amino Acid Change​
p.G12S
Nucleotide Change​
c.34G>A
Wildtype Allele​
C
Mutant Allele​
T
Mutation Strand​
-
Mutant description​
Substitution - Missense
Positive Control Sequence​
AATGATTCTGAATTAGCTGTATCGTCAAGGCACTCTTGCCTACGCCACCAGCTCCAACTACCACAAGTTTATATTCAGTCATTTTCAGCAGGCCTTATAATAAAAATAATG
Amplicon length​
91
Recommended Restriction Enzyme​
CviQI,EcoRI,XbaI,PvuII
Wet-lab validated​
dPCR wet-lab validated
Probe Fluorophore​
FAM/HEX or ATTO550/ROX
Quencher​
Iowa Black
Primer Purification​
Desalted
Probe Purification​
HPLC

Product Resources

icon_0245_cc_gen_pdf-s Validation Data
File Size: 86.13 KBLanguage: English

Resources

Instrument User Manuals (1)
Quick-Start Protocols (1)
Safety Data Sheets (2)
Download Safety Data Sheets for QIAGEN product components.
Certificates of Analysis (1)
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