Assay Name
dPCR Mutation Assay BRAF 477 Human
GeneGlobe Cat No (Assay ID)
DMH0000039
Species
Human (Homo sapiens)
Gene Symbol
BRAF
Gene aliases
BRAF1;BRAF-1
Ensembl Gene ID
ENSG00000157764
Entrez Gene ID
673
Genomic Mutation ID (COSV by COSMIC)
COSV56059623
Legacy Mutation ID (COSM by COSMIC)
COSM477
Amino Acid Change
p.V600D
Nucleotide Change
c.1799_1800delinsAT
Wildtype Allele
TCA
Mutant Allele
TAT
Mutation Strand
-
Mutant description
Substitution - Missense
Positive Control Sequence
GAAAAATAGCCTCAATTCTTACCATCCACAAAATGGATCCAGACAACTGTTCAAACTGATGGGACCCACTCCATCGAGATTTCACTGTAGCTAGACCAAAATCACCTATTTTTACTGTGAGGTCTTCAT
Amplicon length
109
Recommended Restriction Enzyme
CviQI,HaeIII,EcoRI,XbaI,PvuII
Wet-lab validated
dPCR wet-lab validated
Probe Fluorophore
FAM/HEX or ATTO550/ROX
Quencher
Iowa Black
Primer Purification
Desalted
Probe Purification
HPLC