Assay Name
dPCR Mutation Assay EGFR 6239 Human
GeneGlobe Cat No (Assay ID)
DMH0000057
Species
Human (Homo sapiens)
Gene Symbol
EGFR
Gene aliases
ERBB1;ERRP;ERBB
Ensembl Gene ID
ENSG00000146648
Entrez Gene ID
1956
Genomic Mutation ID (COSV by COSMIC)
COSV51769339
Legacy Mutation ID (COSM by COSMIC)
COSM6239
Amino Acid Change
p.G719A
Nucleotide Change
c.2156G>C
Wildtype Allele
G
Mutant Allele
C
Mutation Strand
+
Mutant description
Substitution - Missense
Positive Control Sequence
GGAGAAGCTCCCAACCAAGCTCTCTTGAGGATCTTGAAGGAAACTGAATTCAAAAAGATCAAAGTGCTGGGCTCCGGTGCGTTCGGCACGGTGTATAAGGTAAGGTCCCTGGCA
Amplicon length
94
Recommended Restriction Enzyme
CviQI,HaeIII,XbaI,PvuII
Wet-lab validated
dPCR wet-lab validated
Probe Fluorophore
FAM/HEX or ATTO550/ROX
Quencher
Iowa Black
Primer Purification
Desalted
Probe Purification
HPLC