Configure and order with our online qPCR assay design tools
Products?????? | miRCURY LNA miRNA Custom PCR AssaysFor miRNA quantification using LNA-optimized miRNA qPCR | miRCURY LNA miRNA Custom PCR PanelsFor miRNA profiling analysis using LNA-optimized miRNA qPCR assays | miRCURY LNA miRNA Probe Custom PCR AssaysFor miRNA quantification using LNA-optimized miRNA qPCR |
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Type | Assay | Panel containing any of our 20,000 predesigned assays and/or custom assays | Assay |
Analyte | miRNA | miRNA | miRNA |
LNA Enhanced | |||
Detection Chemistry | SYBR Green | SYBR Green | Hydrolysis probe |
Dynamic Range | Down to 10 miRNA copies | Down to 10 miRNA copies | Down to 1 miRNA copy |
Format | Tube | 96- or 384-well | Tube |
Supported Species | Human, mouse, rat, bacteria & fungi, plants, others | Human, mouse, rat, bacteria & fungi, plants, others | Human, mouse, rat, bacteria & fungi, plants, others |
Data Analysis | False | False | False |
Products?????? | QuantiNova LNA PCR Custom Assays | QuantiNova LNA PCR Custom Panels | QuantiNova LNA PCR Flexible Panels | QuantiNova LNA Probe PCR Custom Assays | QuantiNova LNA Probe PCR Custom Panels | QuantiNova LNA Probe PCR Flexible Panels | Custom RT2 PCR Arrays |
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Type | |||||||
Analyte | |||||||
LNA Enhanced | |||||||
Detection Chemistry | |||||||
Dynamic Range | |||||||
Format | |||||||
Supported Species | |||||||
Data Analysis |
Products?????? | Microbial PCR Custom Array |
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Type | |
Analyte | |
LNA Enhanced | |
Detection Chemistry | SYBR Green |
Dynamic Range | |
Format | |
Supported Species | |
Data Analysis |
Apoptosis is a coordinated, energy-dependent process that involves the activation of a group of cysteine proteases called caspases and a cascade of events that link the initiating stimuli to programmed cell death. Caspases in apoptosis are broadly divided into initiators (caspases 2, 8, 9 and 10) and executioners (caspases 3, 6 and 7). The two main pathways of apoptosis are the intrinsic and extrinsic pathways. Each pathway requires specific triggers to initiate a cascade of molecular events that converge at the stage of caspase 3 activation. The activation of caspase 3 in turn triggers an execution pathway resulting in characteristic cytomorphological features including cell shrinkage, membrane blebbing, chromatin condensation and DNA fragmentation.
The intrinsic signaling pathways that initiate apoptosis involve non-receptor-mediated intracellular signals (e.g. DNA damage, radiation) that cause changes in the inner mitochondrial membrane. The end result is a change in mitochondrial transmembrane potential and release of two main groups of pro-apoptotic proteins from the intermembrane space into the cytosol. The first group consists of cytochrome c (CYTC), SMAC/DIABLO, and the serine protease high temperature requirement protein A2 (HTRA2/OMI). These proteins activate the caspase-dependent mitochondrial pathway. CYTC binds and activates apoptotic peptidase activating factor 1(APAF-1) as well as procaspase-9, forming an apoptosome leading to caspase-9 activation. SMAC/DIABLO and HTRA2/OMI promote apoptosis by inhibiting IAP (inhibitors of apoptosi