Assay Name
dPCR Mutation Assay IDH2 33733 Human
GeneGlobe Cat No (Assay ID)
DMH0000064
Species
Human (Homo sapiens)
Gene Symbol
IDH2
Gene aliases
IDH-2
Ensembl Gene ID
ENSG00000182054
Entrez Gene ID
3418
Genomic Mutation ID (COSV by COSMIC)
COSV57468734
Legacy Mutation ID (COSM by COSMIC)
COSM33733
Amino Acid Change
p.R172K
Nucleotide Change
c.515G>A
Wildtype Allele
C
Mutant Allele
T
Mutation Strand
-
Mutant description
Substitution - Missense
Positive Control Sequence
CCAGTCGGGGGGTGCCCAGGTCAGTGGATCCCCTCTCCACCCTGGCCTACCTGGTCGCCATGGGCGTGCCTGCCAATGGTGATGGGCTTGGTCCAGCCAGGGACT
Amplicon length
85
Recommended Restriction Enzyme
CviQI,AluI,EcoRI,XbaI,PvuII
Wet-lab validated
dPCR wet-lab validated
Probe Fluorophore
FAM/HEX or ATTO550/ROX
Quencher
Iowa Black
Primer Purification
Desalted
Probe Purification
HPLC