GeneGlobe ID: DMH0000240 | Cat. No.: | dPCR LNA Mutation Assays

dPCR Mutation Assay EGFR 6225 Human

AA Mutation: p.E746_A750del
Nucleotide Mutation: c.2236_2250del
dPCR wet-lab validated

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Product Specification

Assay Name​
dPCR Mutation Assay EGFR 6225 Human
GeneGlobe Cat No (Assay ID)​
DMH0000240
Species​
Human (Homo sapiens)
Gene Symbol​
EGFR
Gene aliases​
ERBB1;ERRP;ERBB
Ensembl Gene ID​
ENSG00000146648
Entrez Gene ID​
1956
Genomic Mutation ID (COSV by COSMIC)​
COSV51765066
Legacy Mutation ID (COSM by COSMIC)​
COSM6225
Amino Acid Change​
p.E746_A750del
Nucleotide Change​
c.2236_2250del
Wildtype Allele​
GGAATTAAGAGAAGCA
Mutant Allele​
G
Mutation Strand​
+
Mutant description​
Substitution - Missense
Positive Control Sequence​
CATAGGGACTCTGGATCCCAGAAGGTGAGAAAGTTAAAATTCCCGTCGCTATCAAGGAATTAAGAGAAGCAACATCTCCGAAAGCCAACAAGGAAATCCTCGATGTGAGTTTCTGCTTTG
Amplicon length​
100
Recommended Restriction Enzyme​
CviQI,AluI,HaeIII,EcoRI,XbaI,PvuII
Wet-lab validated​
dPCR wet-lab validated
Probe Fluorophore​
FAM/HEX or ATTO550/ROX
Quencher​
Iowa Black
Primer Purification​
Desalted
Probe Purification​
HPLC

Product Resources

Validation Data
File Size: 125.75 KBLanguage: English

Resources

Instrument User Manuals (1)
Quick-Start Protocols (1)
Safety Data Sheets (2)
Download Safety Data Sheets for QIAGEN product components.
Certificates of Analysis (1)
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