Assay Name
dPCR Mutation Assay PTEN 5154 Human
GeneGlobe Cat No (Assay ID)
DMH0000295
Species
Human (Homo sapiens)
Gene Symbol
PTEN
Gene aliases
MMAC1;TEP1;PTEN1;BZS;MHAM
Ensembl Gene ID
ENSG00000171862
Entrez Gene ID
5728
Genomic Mutation ID (COSV by COSMIC)
COSV64288653
Legacy Mutation ID (COSM by COSMIC)
COSM5154
Amino Acid Change
p.R233*
Nucleotide Change
c.697C>T
Wildtype Allele
C
Mutant Allele
T
Mutation Strand
+
Mutant description
Substitution - Missense
Positive Control Sequence
TCCTCAGTTTGTGGTCTGCCAGCTAAAGGTGAAGATATATTCCTCCAATTCAGGACCCACACGACGGGAAGACAAGTTCATGTACTTTGAGTTCCCTCAGCCGTTACCTGTGTGT
Amplicon length
95
Recommended Restriction Enzyme
HaeIII,EcoRI,XbaI,PvuII
Wet-lab validated
dPCR wet-lab validated
Probe Fluorophore
FAM/HEX or ATTO550/ROX
Quencher
Iowa Black
Primer Purification
Desalted
Probe Purification
HPLC